Predictive value of cytoplasmic granulation patterns during in vitro fertilization in metaphase II oocytes: Part I, poor-prognosis patients

ObjectiveTo determine whether 4 cytoplasmic granulation patterns of human metaphase II oocytes have a predictive value for in vitro fertilization outcomes.DesignA retrospective cohort study.SettingAn academically affiliated private clinical infertility and research center.Patient(s)A total 2,690 consecutive fresh autologous collected from women aged 41.2 ± 5.0 years between 2017 2019.Intervention(s)Determination pattern every oocyte during intracytoplasmic sperm injection as fine, central, dispersed, newly introduced uneven granulations.Main Outcome Measure(s)Fertilization outcomes (2 pronuclei [2PN], <2PN, >2PN rates), pregnancy, live birth rates different at ages.Result(s)Fine produced the highest 2PN rate, followed by uneven, dispersed (91.8%, 83.9%, 77.9%, 54.8%, respectively). Differences were surprisingly relatively independent age other variables. Overall, compared with fine granulation, resulted lower pregnancy (4.6% vs. 10.7%) known-outcome analysis (1.3% 5.6%) well (3.0% 8.9%) (0.6% 5.6%). The demonstrated that had than (2.3% Unexpectedly, ooplasm largely disassociated embryo morphologic grades.Conclusion(s)We, first time, distinct had, variables, fertilization, cycles poor-prognosis patients. These data suggest upstream deserve closer attention terms selection. To outcomes. A study. An center. 2019. Determination granulations. Fertilization ages. Fine grades. We,